Overview

  • Product name
  • Description
    Rabbit polyclonal to CLIC1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CLIC1 protein

  • Positive control
    • A375 cell line lysates; human prostate carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab76592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 27 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function
    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificity
    Expression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similarities
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localization
    Nucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

Images

  • Anti-CLIC1 antibody (ab76592) at 1/60 dilution + A375 cell line lysates at 35 µg

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • ab76592, at a 1/10 dilution, staining CLIC1 in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry. ab76592 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References

ab76592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y)
Permeabilization
No
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Methanol
Username

Anu Shanu

Verified customer

Submitted Jan 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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