Overview

  • Product nameAnti-CLIC1 antibody
    See all CLIC1 primary antibodies
  • Description
    Rabbit polyclonal to CLIC1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CLIC1 protein

  • Positive control
    • A375 cell line lysates; human prostate carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab76592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 27 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificityExpression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localizationNucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

Anti-CLIC1 antibody images

  • Anti-CLIC1 antibody (ab76592) at 1/60 dilution + A375 cell line lysates at 35 µg

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • ab76592, at a 1/10 dilution, staining CLIC1 in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry. ab76592 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-CLIC1 antibody (ab76592)

ab76592 has not yet been referenced specifically in any publications.

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