Overview

  • Product name
  • Description
    Mouse monoclonal to CLIC1
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-242 of Human CLIC1

  • Positive control
    • HL-60 (human promyelocytic leukemia) whole cell lysate
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab77214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 27 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificity
    Expression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similarities
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localization
    Nucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

Images

  • ab77214 staining CLIC1 in the 184A1 HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were incubated with primary antibody at a concentration of 10 ug/mL.

  • ab77214 at 3ug/ml, staining CLIC1 by immunohistochemistry on formalin-fixed paraffin-embedded human colon tissue.
  • Anti-CLIC1 antibody (ab77214) at 1/2500 dilution + Recombinant peptide used as the immunogen at 0.1 µg

    Predicted band size : 27 kDa
    Quality Control Testing: Antibody Reactive Against Recombinant Protein.
  • Anti-CLIC1 antibody (ab77214) at 1/1000 dilution + HL-60 cell lysate at 25 µg

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Overlay histogram showing THP1 cells stained with ab77214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Ding Q  et al. CLIC1 overexpression is associated with poor prognosis in gallbladder cancer. Tumour Biol N/A:N/A (2014). Read more (PubMed: 25227665) »
  • Lomnytska MI  et al. Impact of genomic stability on protein expression in endometrioid endometrial cancer. Br J Cancer 106:1297-305 (2012). WB ; Human . Read more (PubMed: 22415234) »

See all 4 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab77214.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up