Overview

  • Product nameAnti-CLIC2 antibody
    See all CLIC2 primary antibodies
  • Description
    Rabbit polyclonal to CLIC2
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from a region between amino acids 162-211 of human CLIC2. (Note: the exact amino acid sequence is proprietary).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
  • Tissue specificityDetected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localizationCytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 2 antibody
    • Chloride intracellular channel protein 2 antibody
    • CLIC 2b antibody
    • CLIC2 antibody
    • CLIC2_HUMAN antibody
    • CLIC2b antibody
    • MRXS32 antibody
    • OTTHUMP00000196471 antibody
    • XAP 121 antibody
    • XAP121 antibody
    see all

Anti-CLIC2 antibody images

  • Anti-CLIC2 antibody (ab28694) at 1.25 µg/ml + HepG2 cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-CLIC2 antibody (ab28694)

ab28694 has not yet been referenced specifically in any publications.

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