Overview

  • Product name
  • Description
    Rabbit polyclonal to CLIC3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-220 of Human CLIC3 (NP_004660)

  • Positive control
    • HeLaS3 lysate Ca922; T(IV)(lung CA) (IHC)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100.

Target

  • Relevance
    Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.
  • Cellular localization
    Nuclear, cytoplasmic and Single pass membrane. Predominantly nuclear but also found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain
  • Database links
  • Alternative names
    • 2300003G24Rik antibody
    • Chloride intracellular channel 3 antibody
    • Chloride intracellular channel protein 3 antibody
    • MGC109225 antibody
    • OTTHUMP00000022630 antibody
    see all

Images

  • Anti-CLIC3 antibody (ab97289) at 1/500 dilution + HeLaS3 whole cell lysate at 30 µg

    Predicted band size : 27 kDa
  • Immunohistochemical analysis of paraffin embedded T(IV)(lung CA), using ab97289 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Ca922, using ab97289 at 1/100 dilution.

References

ab97289 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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