Overview

  • Product name
  • Description
    Rabbit polyclonal to CLIC4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 3 and 253 of Human CLIC4 (Uniprot ID: Q9Y696)

  • Positive control
    • HeLa and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab153836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • Tissue specificity
    Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • Sequence similarities
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Images

  • Anti-CLIC4 antibody (ab153836) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab153836 antibody (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled in red.

References

ab153836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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