Overview

  • Product nameAnti-CLIC4 antibody
    See all CLIC4 primary antibodies
  • Description
    Rabbit polyclonal to CLIC4
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CLIC4 protein

  • Positive control
    • CLIC4 transfected 293 cell lysates; human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab76593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 29 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • FunctionCan insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • Tissue specificityDetected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 4 antibody
    • Chloride intracellular channel 4 (mitochondrial) antibody
    • Chloride intracellular channel 4 like antibody
    • Chloride intracellular channel protein 4 antibody
    • Clic4 antibody
    • CLIC4_HUMAN antibody
    • CLIC4L antibody
    • DKFZP566G223 antibody
    • FLJ38640 antibody
    • H1 antibody
    • HUH1 antibody
    • Intracellular chloride ion channel protein p64H1 antibody
    • MC3S5 antibody
    • mtCLIC antibody
    • p64H1 antibody
    see all

Anti-CLIC4 antibody images

  • All lanes : Anti-CLIC4 antibody (ab76593) at 1/50 dilution

    Lane 1 : Non transfected 293 cell lysates
    Lane 2 : CLIC4 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab76593 at a 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-CLIC4 antibody (ab76593)

ab76593 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (neonatal cardiomyocytes)
Specification neonatal cardiomyocytes
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"