• Product name
  • Description
    Rabbit polyclonal to CLIC5
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: PFLTFNGDVK TDVNKIEEFL EETLTPEKYP KLAAKHRESN TAGIDIFSKF, corresponding to N terminal amino acids 73-122 of Human CLIC5

  • Positive control
    • Jurkat cell lysate. Human intestine.



Our Abpromise guarantee covers the use of ab66630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.


  • Function
    Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture.
  • Tissue specificity
    Isoform 1: Expressed in renal glomeruli endothelial cells and podocytes (at protein level).
  • Sequence similarities
    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • Domain
    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Membrane. Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain and Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Membrane. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). Colocalized with podocalyxin in renal glomeruli.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 5 antibody
    • Chloride intracellular channel 5A antibody
    • Chloride intracellular channel protein 5 antibody
    • CLIC 5 antibody
    • CLIC 5B antibody
    • CLIC5 antibody
    • CLIC5_HUMAN antibody
    • CLIC5B antibody
    • dJ447E21.4 antibody
    • FLJ90663 antibody
    • MST 130 antibody
    • MST130 antibody
    • MSTP 130 antibody
    • MSTP130 antibody
    see all

Anti-CLIC5 antibody images

  • Anti-CLIC5 antibody (ab66630) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling CLIC5 with ab66630 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

References for Anti-CLIC5 antibody (ab66630)

ab66630 has not yet been referenced specifically in any publications.

Product Wall

Western blot
Mouse Tissue lysate - whole (cardiac homogenates)
Loading amount
10 µg
cardiac homogenates
Gel Running Conditions
Reduced Denaturing (10.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Oct 30 2008


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