Overview

  • Product nameAnti-CLIC5 antibody
    See all CLIC5 primary antibodies
  • Description
    Rabbit polyclonal to CLIC5
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CLIC5 protein

  • Positive control
    • K562 cell line lysate. Human colon carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesab75948 is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 47 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • FunctionCan insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture.
  • Tissue specificityIsoform 1: Expressed in renal glomeruli endothelial cells and podocytes (at protein level).
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Membrane. Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain and Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Membrane. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity). Colocalized with podocalyxin in renal glomeruli.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride intracellular channel 5 antibody
    • Chloride intracellular channel 5A antibody
    • Chloride intracellular channel protein 5 antibody
    • CLIC 5 antibody
    • CLIC 5B antibody
    • CLIC5 antibody
    • CLIC5_HUMAN antibody
    • CLIC5B antibody
    • dJ447E21.4 antibody
    • FLJ90663 antibody
    • MST 130 antibody
    • MST130 antibody
    • MSTP 130 antibody
    • MSTP130 antibody
    see all

Anti-CLIC5 antibody images

  • Anti-CLIC5 antibody (ab75948) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab75948, at 1/10 dilution, staining CLIC5 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry. ab75948 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-CLIC5 antibody (ab75948)

ab75948 has not yet been referenced specifically in any publications.

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