The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport.
Tissue specificityExpressed in brain, placenta, pancreas and liver.
Sequence similaritiesBelongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain.
DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
Cellular localizationCytoplasm. Cell membrane. Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane.