Overview

  • Product nameAnti-CLINT1 antibody
    See all CLINT1 primary antibodies
  • Description
    Mouse monoclonal to CLINT1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: GVSSDSVGGF RYSERYDPEP KSKWDEEWDK NKSAFPFSDK LGELSDKIGS TIDDTISKF RRKDREDSPE RCSDSDEEKK ARRGRSPKGE FKDEEETVTTK H, corresponding to amino acids 161-261 of Human CLINT1, (NP_055481), with a 26 kDa enzyme tag.

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 68 kDa.

Target

  • FunctionBinds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
  • Tissue specificityUbiquitously expressed at low to intermediate levels.
  • Sequence similaritiesBelongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Clathrin interacting protein antibody
    • Clathrin interactor 1 antibody
    • Clathrin-interacting protein localized in the trans-Golgi region antibody
    • CLINT 1 antibody
    • Clint antibody
    • CLINT1 antibody
    • ENTH antibody
    • Enthoprotin antibody
    • EPN4 antibody
    • EPN4_HUMAN antibody
    • Epsin 4 antibody
    • Epsin related protein antibody
    • Epsin-4 antibody
    • Epsin-related protein antibody
    • EpsinR antibody
    see all

Anti-CLINT1 antibody images

  • Anti-CLINT1 antibody (ab76482) at 5 µg/ml + recombinant CLINT1 fragment with 26 kDa tag at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 68 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-CLINT1 antibody (ab76482) at 5 µg/ml + Jurkat cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)

References for Anti-CLINT1 antibody (ab76482)

ab76482 has not yet been referenced specifically in any publications.

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