FunctionBinds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
Tissue specificityUbiquitously expressed at low to intermediate levels.
Sequence similaritiesBelongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
Clathrin-interacting protein localized in the trans-Golgi region antibody
CLINT 1 antibody
Epsin 4 antibody
Epsin related protein antibody
Epsin-related protein antibody
Anti-CLINT1 antibody images
Western blot - CLINT1 antibody (ab86046)
All lanes : Anti-CLINT1 antibody (ab86046) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of Human CLINT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86046 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-CLINT1 antibody (ab86046)
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