Overview

  • Product name
  • Description
    Rabbit polyclonal to CLINT1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 250 and 300 of Human CLINT1 (NP_055481.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab86046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
  • Tissue specificity
    Ubiquitously expressed at low to intermediate levels.
  • Sequence similarities
    Belongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Clathrin interacting protein antibody
    • Clathrin interactor 1 antibody
    • Clathrin-interacting protein localized in the trans-Golgi region antibody
    • CLINT 1 antibody
    • Clint antibody
    • CLINT1 antibody
    • ENTH antibody
    • Enthoprotin antibody
    • EPN4 antibody
    • EPN4_HUMAN antibody
    • Epsin 4 antibody
    • Epsin related protein antibody
    • Epsin-4 antibody
    • Epsin-related protein antibody
    • EpsinR antibody
    see all

Images

  • All lanes : Anti-CLINT1 antibody (ab86046) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of Human CLINT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86046 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab86046 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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