Anti-CLINT1 antibody (ab86046)
Key features and details
- Rabbit polyclonal to CLINT1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CLINT1 antibody
See all CLINT1 primary antibodies -
Description
Rabbit polyclonal to CLINT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to a region between residues 250 and 300 of Human CLINT1 (NP_055481.1).
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Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 68 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly. -
Tissue specificity
Ubiquitously expressed at low to intermediate levels. -
Sequence similarities
Belongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network. - Information by UniProt
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Database links
- Entrez Gene: 538540 Cow
- Entrez Gene: 9685 Human
- Entrez Gene: 216705 Mouse
- Entrez Gene: 360515 Rat
- Omim: 607265 Human
- SwissProt: A7Z035 Cow
- SwissProt: Q14677 Human
- SwissProt: Q99KN9 Mouse
see all -
Alternative names
- Clathrin interacting protein antibody
- Clathrin interactor 1 antibody
- Clathrin-interacting protein localized in the trans-Golgi region antibody
see all
Images
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All lanes : Anti-CLINT1 antibody (ab86046) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
Detection of Human CLINT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86046 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86046 has not yet been referenced specifically in any publications.