Overview

  • Product nameAnti-CLIP3 antibody
    See all CLIP3 primary antibodies
  • Description
    Rabbit polyclonal to CLIP3
  • SpecificityThis antibody detects endogenous levels of total CLIP3 protein.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from the internal region of human CLIP3.

  • Positive control
    • A549 cells and extracts from A549 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

Target

  • FunctionFunctions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 2 CAP-Gly domains.
  • DomainMicrotubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).
  • Cellular localizationCytoplasm. Golgi apparatus > Golgi stack. Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500005P14Rik antibody
    • AI844915 antibody
    • CAP GLY domain containing linker protein 3 antibody
    • CAP-Gly domain-containing linker protein 3 antibody
    • CLIP 170 related 59 kDa protein antibody
    • CLIP-170-related 59 kDa protein antibody
    • clip3 antibody
    • CLIP3_HUMAN antibody
    • CLIPR 59 antibody
    • CLIPR-59 antibody
    • CLIPR59 antibody
    • Cytoplasmic linker protein 170 related 59 kDa protein antibody
    • Cytoplasmic linker protein 170-related 59 kDa protein antibody
    • Restin like 1 antibody
    • RSNL1 antibody
    see all

Anti-CLIP3 antibody images

  • All lanes : Anti-CLIP3 antibody (ab74239) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from A549 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Immunofluorescence analysis of A549 cells using ab74239 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References for Anti-CLIP3 antibody (ab74239)

ab74239 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 02 2016

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 02 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% triton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 18 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% triton x-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"