Overview

  • Product nameAnti-CLK1 antibody
    See all CLK1 primary antibodies
  • Description
    Rabbit polyclonal to CLK1
  • SpecificityThis antibody detects endogenous levels of total CLK1 protein.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from the internal region of human CLK1.

  • Positive control
    • HUVEC cells and extracts from 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionPhosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylates on all three types of residues.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC like kinase 1 antibody
    • CDC-like kinase 1 antibody
    • CDC28/CDC2 like kinase antibody
    • CLK antibody
    • CLK/STY antibody
    • Clk1 antibody
    • CLK1_HUMAN antibody
    • Dual specificity protein kinase CLK1 antibody
    • Protein tyrosine kinase STY antibody
    • STY antibody
    see all

Anti-CLK1 antibody images

  • All lanes : Anti-CLK1 antibody (ab74044) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 30 kDa,34 kDa,36 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of CLK1 in HUVEC cells, using ab74044 at a 1/500 dilution.
    Left image: untreated.
    Right image: treated with immunizing peptide.

References for Anti-CLK1 antibody (ab74044)

This product has been referenced in:
  • Ninomiya K  et al. Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor. J Cell Biol 195:27-40 (2011). WB . Read more (PubMed: 21949414) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (P19 cells)
Gel Running Conditions Non-reduced Denaturing (4 - 12 % Novex gel with Mops runing buffer)
Loading amount 30 µg
Specification P19 cells
Blocking step BSA as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 05 2016

The amino acid range I gave you before, 110 - 160, is not the ab74044 immunogen, it is just a range that contains it. The exact sequence is considered proprietary by the developer of the antibody, but I was at least able to find out that the immunogen ...

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Here is the IF protocol from the lab that validated this antibody for this application. The "polyoxymethylene" is 4% paraformaldehyde. The 24-well plates that are referred to in the protocol are either cover-slip-bottomed or contain cover-slips. Chambe...

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The CLK homology information I received from our sources of the two CLK1 antibodies and the one CLK4 antibody that will be useful for western blotting of human samples is as follows. The percentages refer to percentage identity of amino acids of the an...

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Thank you for contacting us. I am very sorry for the confusion. I performed a BLAST to find out the percentage of homology between the four proteins, and the results are: -CLK1-CLK2: 53.5% -CLK1-CLK3: 14% -CLK1-CLK4: 77% -CLK2-CL...

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Thank you for your inquiry. Unfortunately, we do not currently have more information about ab74044 citations in publications. We will update the on-line datasheet for this product as soon as we receive additional data. We however can gu...

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Thank you for your email. I apologize for being late in getting back to you as our lab took more time to release this information than initially anticipated. They have let me know that the antibody is specific to CLK1 only. I hope this infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"