Anti-CLK2 antibody (ab188141)
Key features and details
- Rabbit polyclonal to CLK2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLK2 antibody
See all CLK2 primary antibodies -
Description
Rabbit polyclonal to CLK2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human CLK2 aa 1-100.
Database link: P49760 -
Positive control
- IHC-P: Human colon, testis, and skin tissues; WB: BeWo cell lysate; ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylates on all three types of residues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1196 Human
- Entrez Gene: 12748 Mouse
- Omim: 602989 Human
- SwissProt: P49760 Human
- SwissProt: O35491 Mouse
- Unigene: 73986 Human
- Unigene: 288098 Mouse
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Alternative names
- CDC like kinase 2 antibody
- CDC-like kinase 2 antibody
- CLK 2 antibody
see all
Images
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Anti-CLK2 antibody (ab188141) at 0.4 µg/ml + BeWo (human placenta choriocarcinoma cell line) cell lysate
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling CLK2 with ab188141 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of PFA/Triton X-100 fixed U-2 OS cells labeling CLK2 with ab188141 at 2 µg/ml.
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Paraffin embedded human colon tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.
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Paraffin embedded human skin tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.
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Paraffin embedded human skeletal muscle tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in myocytes cells as expected.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab188141 has been referenced in 3 publications.
- Park SY et al. Depletion of CLK2 sensitizes glioma stem-like cells to PI3K/mTOR and FGFR inhibitors. Am J Cancer Res 10:3765-3783 (2020). PubMed: 33294266
- Kulkarni P et al. Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity. Proc Natl Acad Sci U S A 114:E2644-E2653 (2017). PubMed: 28289210
- Park SY et al. Cdc2-like kinase 2 is a key regulator of the cell cycle via FOXO3a/p27 in glioblastoma. Oncotarget 7:26793-805 (2016). PubMed: 27050366