Overview

  • Product nameAnti-CLK2 antibody
    See all CLK2 primary antibodies
  • Description
    Rabbit polyclonal to CLK2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Chinese Hamster
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CLK2.

    (Peptide available as ab99082.)

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionPhosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylates on all three types of residues.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC like kinase 2 antibody
    • CDC-like kinase 2 antibody
    • CLK 2 antibody
    • CLK kinase antibody
    • CLK2 antibody
    • Clk2 Scamp3 antibody
    • CLK2_HUMAN antibody
    • Dual specificity protein kinase CLK2 antibody
    • hCLK 2 antibody
    • hCLK2 antibody
    • Tu52 antibody
    see all

Anti-CLK2 antibody images

  • ab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 36 kDa,64 kDa,94 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa).
  • ICC/IF image of ab86147 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86147 at 5ug overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed cell types at 5ug/ml

References for Anti-CLK2 antibody (ab86147)

This product has been referenced in:
  • Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). Read more (PubMed: 27126587) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - whole cell (Colon carcinoma cells)
Specification Colon carcinoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Apr 18 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
Specification Chinese Hamster Ovary cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Apr 02 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Hamster Cell (Chinese Hamster Ovary cells)
Specification Chinese Hamster Ovary cells
Permeabilization Yes - 0.5% Triton
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Apr 01 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Human Cell (Colon carcinoma cells)
Specification Colon carcinoma cells
Permeabilization No
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Mar 24 2014

I am very pleased to hear you would like to accept our offer and test ab65082 and/or ab86147 in immunoprecipitation. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immun...

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