Overview

  • Product name
  • Description
    Rabbit polyclonal to CLK4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (RSRRSSPKRK RNRHCSSHQS RSKSHRRKRS RSIEDDEEGH LICQSGDVLR) of Human CLK4 (NP_065717).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
  • Tissue specificity
    Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylates on all three types of residues.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI987988 antibody
    • C85119 antibody
    • CDC like kinase 4 antibody
    • CDC-like kinase 4 antibody
    • CLK4 antibody
    • CLK4_HUMAN antibody
    • DKFZp686A20267 antibody
    • Dual specificity protein kinase CLK4 antibody
    • OTTMUSP00000005756; antibody
    • OTTMUSP00000005757 antibody
    • OTTMUSP00000041113 antibody
    • Protein serine threonine kinase Clk4 antibody
    • RP23-9N7.1 antibody
    see all

Images

  • Anti-CLK4 antibody (ab104321) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size : 57 kDa


  • Predicted band size : 57 kDa

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Permeabilization
No
Specification
Colon carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Methanol
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Jul 16 2015

The CLK homology information I received from our sources of the two CLK1 antibodies and the one CLK4 antibody that will be useful for western blotting of human samples is as follows. The percentages refer to percentage identity of amino acids of the an...

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