Overview

  • Product name
  • Description
    Rabbit polyclonal to CLLD7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-244 of Human CLLD7 (UniProt: Q8NDN9).

  • Positive control
    • NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain whole cell lysates; MCF7 cells; Human J5 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May be involved in cell cycle regulation by chromatin remodeling.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 2 BTB (POZ) domains.
    Contains 6 RCC1 repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chronic lymphocytic leukemia deletion region gene 7 protein antibody
    • CLL deletion region gene 7 protein antibody
    • CLLL7 antibody
    • CLLL7 protein antibody
    • E4.5 antibody
    • GDP/GTP exchange factor (GEF) like protein antibody
    • GLP antibody
    • Guanine nucleotide exchange factor antibody
    • Hypothetical protein FLJ39335 antibody
    • RCBT1_HUMAN antibody
    • Rcbtb1 antibody
    • RCC1 and BTB domain-containing protein 1 antibody
    • Regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 1 antibody
    • Regulator of chromosome condensation and BTB domain-containing protein 1 antibody
    • RP11-185C18.1 antibody
    see all

Images

  • Anti-CLLD7 antibody (ab154649) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    12% SDS PAGE
  • Anti-CLLD7 antibody (ab154649) at 1/2000 dilution + Mouse brain whole cell lysate
    at 50 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed MCF7 cells labeling CLLD7 with ab154649 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human J5 xenograft tissue labeling CLLD7 with ab154649 at 1/500 dilution.

References

ab154649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Lymphoblastoid cell lines (LCLs))
Total protein in input
30 µg
Immuno-precipitation step
Protein A/G
Specification
Lymphoblastoid cell lines (LCLs)
Username

Abcam user community

Verified customer

Submitted Sep 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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