Anti-CLLD8/SETDB2 antibody (ab13712)

Overview

  • Product name
    Anti-CLLD8/SETDB2 antibody
    See all CLLD8/SETDB2 primary antibodies
  • Description
    Goat polyclonal to CLLD8/SETDB2
  • Specificity
    This antibody has been shown to detect SETDB2 in overexpression cell extracts.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CLLD8/SETDB2 aa 2-14 (N terminal).
    Sequence:

    GEKNGDAKTFWME-C


    Database link: Q96T68
    (Peptide available as ab23232)

  • General notes
    GenBank Accession Number - NP_114121

Properties

Applications

Our Abpromise guarantee covers the use of ab13712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human CLLD8/SETDB2 peptide (ab23232).

Target

  • Function
    Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
  • Tissue specificity
    Ubiquitous. Highest expression in heart, testis and ovary.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf4 antibody
    • Chromosome 13 open reading frame 4 antibody
    • Chronic lymphocytic leukemia deletion region 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 antibody
    • Chronic lymphocytic leukemia deletion region gene 8 protein antibody
    • Clld8 antibody
    • CLLL8 antibody
    • Gm293 antibody
    • H3-K9-HMTase antibody
    • Histone H3-K9 methyltransferase antibody
    • Histone-lysine N-methyltransferase SETDB2 antibody
    • KMT1F antibody
    • Lysine N-methyltransferase 1F antibody
    • Probable histone-lysine N-methyltransferase H3 lysine-9 specific antibody
    • SEB2 antibody
    • SET domain bifurcated 2 antibody
    • SET domain protein, bifurcated, 2 antibody
    • SETB2_HUMAN antibody
    • Setdb2 antibody
    see all

Images

  • Anti-CLLD8/SETDB2 antibody (ab13712) at 0.5 µg/ml (Staining of Human Heart lysate)

    Predicted band size : 83 kDa

References

ab13712 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your inquiry. I BLASTed the immunogen sequence of ab5517 against mouse and it only has 55% homology.  We generally say that anything with a greater than 85% homology would be expected to cross-react, so this is quite low.  This antibo...

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I'm very sorry to hear you are having problems with ab5517 and ab13712. You do not mention adding loading buffer to your samples prior to boiling, please make sure you add loading buffer containing SDS, DTT, beta-mercaptoethanol and bromphenol blue...

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Thank you for your enquiry. Currently we don't have either the lysate or the clone for this antibody within our catalogue. In the future, if you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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