Overview

  • Product name
    Anti-CLLD8/SETDB2 antibody
    See all CLLD8/SETDB2 primary antibodies
  • Description
    Rabbit polyclonal to CLLD8/SETDB2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CLLD8/SETDB2 (N terminal).
    Database link: Q96T68

Properties

Applications

Our Abpromise guarantee covers the use of ab56184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
    • Tissue specificity
      Ubiquitous. Highest expression in heart, testis and ovary.
    • Sequence similarities
      Belongs to the histone-lysine methyltransferase family.
      Contains 1 MBD (methyl-CpG-binding) domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • C13orf4 antibody
      • Chromosome 13 open reading frame 4 antibody
      • Chronic lymphocytic leukemia deletion region 8 antibody
      • Chronic lymphocytic leukemia deletion region gene 8 antibody
      • Chronic lymphocytic leukemia deletion region gene 8 protein antibody
      • Clld8 antibody
      • CLLL8 antibody
      • Gm293 antibody
      • H3-K9-HMTase antibody
      • Histone H3-K9 methyltransferase antibody
      • Histone-lysine N-methyltransferase SETDB2 antibody
      • KMT1F antibody
      • Lysine N-methyltransferase 1F antibody
      • Probable histone-lysine N-methyltransferase H3 lysine-9 specific antibody
      • SEB2 antibody
      • SET domain bifurcated 2 antibody
      • SET domain protein, bifurcated, 2 antibody
      • SETB2_HUMAN antibody
      • Setdb2 antibody
      see all

    Images

    • ab56184 staining CLLD8/SETDB2 in 293T cells by Immunocytochemistry/ Immunofluorescence.
      After 2–6 days, the cells grown on coverslips were washed and fixed with 4% paraformaldehyde for 10 minutes, permeabilized, and incubated with ab56184 at a 1/50 dilution for 1 hour at 37 °C. A secondary antibody directed against the rabbit primary antibody conjugated with Alexa-Fluor 488 was used for detection. The nuclei were counterstained with DAPI.

    References

    This product has been referenced in:
    • Falandry C  et al. CLLD8/KMT1F is a lysine methyltransferase that is important for chromosome segregation. J Biol Chem 285:20234-41 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20404330) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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