The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHistone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
Tissue specificityUbiquitous. Highest expression in heart, testis and ovary.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Contains 1 MBD (methyl-CpG-binding) domain. Contains 1 pre-SET domain. Contains 1 SET domain.
Probable histone-lysine N-methyltransferase H3 lysine-9 specific antibody
SET domain bifurcated 2 antibody
SET domain protein, bifurcated, 2 antibody
Anti-CLLD8/SETDB2 antibody images
Immunocytochemistry/ Immunofluorescence - CLLD8/SETDB2 antibody (ab56184)Image from Falandry C et al, J Biol Chem. 2010 Jun 25;285(26):20234-41. Epub 2010 Apr 19, Fig 1. DOI 10.1074/jbc.M109.052399
ab56184 staining CLLD8/SETDB2 in 293T cells by Immunocytochemistry/ Immunofluorescence. After 2–6 days, the cells grown on coverslips were washed and fixed with 4% paraformaldehyde for 10 minutes, permeabilized, and incubated with ab56184 at a 1/50 dilution for 1 hour at 37 °C. A secondary antibody directed against the rabbit primary antibody conjugated with Alexa-Fluor 488 was used for detection. The nuclei were counterstained with DAPI.
References for Anti-CLLD8/SETDB2 antibody (ab56184)
This product has been referenced in:
Falandry C et al. CLLD8/KMT1F is a lysine methyltransferase that is important for chromosome segregation. J Biol Chem285:20234-41 (2010).
Read more (PubMed: 20404330) »