Overview

  • Product name
    Anti-CLMN antibody
  • Description
    Rabbit polyclonal to CLMN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 446-553 of Human CLMN.

  • Positive control
    • Human liver lysate; Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Widely expressed at intermediate level.
  • Sequence similarities
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9330188N17Rik antibody
    • AI428889 antibody
    • AI788815 antibody
    • Calmin antibody
    • Calponin-like transmembrane domain protein antibody
    • CLMN antibody
    • CLMN_HUMAN antibody
    • KIAA1188 antibody
    • mKIAA1188 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121373 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121373 at 1/100 dilution staining CLMN in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Fukuzawa R  et al. The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour. PLoS One 12:e0186333 (2017). Mouse . Read more (PubMed: 29040332) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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