Overview

  • Product name
  • Description
    Rabbit polyclonal to CLN6
  • Specificity
    ab75007 detects endogenous levels of total CLN6 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal region of human CLN6.

  • Positive control
    • WB: extracts from HeLa cells IHC-P: human cervix tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    Defects in CLN6 are the cause of variant late onset infantile neuronal ceroid lipofuscinosis (vLINCL).
  • Cellular localization
    Endoplasmic reticulum membrane; Multi pass membrane protein.
  • Database links
  • Alternative names
    • Ceroid lipofuscinosis, neuronal 6, late infantile, variant antibody
    • CLN6 protein antibody
    • FLJ20561 antibody
    • HsT18960 antibody
    • Nclf antibody
    • Protein CLN6 antibody
    see all

Anti-CLN6 antibody images

  • All lanes : Anti-CLN6 antibody (ab75007) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 28 kDa,30 kDa,78 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of CLN6 in paraffin embedded human cervix tissue using ab75007 at 1/50 dilution with (right image) or without (left image) the immunising peptide.

References for Anti-CLN6 antibody (ab75007)

ab75007 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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