Overview

  • Product nameAnti-CLN6 antibody
    See all CLN6 primary antibodies
  • Description
    Rabbit polyclonal to CLN6
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal region amino acids 252-301 (RLFLDSNGLF LFSSFALTLL LVALWVAWLW NDPVLRKKYP GVIYVPEPWA) of Human CLN6 (NP_060352)

  • Positive control
    • 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-CLN6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling CLN6 with ab82786 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CLN6. Right - Merge.
  • Anti-CLN6 antibody (ab82786) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CLN6 antibody (ab82786)

ab82786 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82786.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"