Overview

  • Product name
    Anti-CLNK antibody
  • Description
    Rabbit polyclonal to CLNK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 280-308 of Human CLNK (NP_443196.2).

  • Positive control
    • HepG2 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.

Target

  • Function
    Plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigen receptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Involved in phosphorylation of LAT.
  • Sequence similarities
    Contains 1 SH2 domain.
  • Domain
    The N-terminal proline-rich region interacts with the SH3 domain of PLCG1.
    The SH2 domain is important for restoration of BCR-induced calcium response and JNK2 activation in BLNK-deficient DT40 cells expressing LAT.
  • Post-translational
    modifications
    Tyrosine-phosphorylated upon BCR cross-linking. Tyrosine phosphorylation at both Tyr-69 and Tyr-96 are required for BCR-induced calcium response and are essential to restore PLCG2-mediated signaling in BLNK-deficient DT40 cells, but this phosphorylation is dispensable in cells expressing LAT. Interacts with the SH2 domain of PLCG1 via phosphorylated Tyr-96.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLNK antibody
    • CLNK_HUMAN antibody
    • Cytokine-dependent hematopoietic cell linker antibody
    • Mast cell immunoreceptor signal transducer antibody
    • MGC159342, antibody
    • MGC35197 antibody
    • MIST antibody
    • RGD1562417 antibody
    see all

Images

  • Anti-CLNK antibody (ab116287) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size: 50 kDa

References

ab116287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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