Anti-Clostridium botulinum A Toxoid antibody [2A33] (ab82709)

Overview

  • Product name
    Anti-Clostridium botulinum A Toxoid antibody [2A33]
    See all Clostridium botulinum A Toxoid primary antibodies
  • Description
    Mouse monoclonal [2A33] to Clostridium botulinum A Toxoid
  • Specificity
    ab82709 reacts with Clostridium botulinum Toxin A heavy chain.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Clostridium botulinum.
  • Immunogen

    Synthetic peptide:

    YIKNIINTSILN LRYESNH

    , corresponding to amino acid region 869-887 of Clostridium botulinum Toxin A.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab82709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • Alternative names
    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    see all

References for Anti-Clostridium botulinum A Toxoid antibody [2A33] (ab82709)

ab82709 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Loading amount
5 µg
Specification
cell lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. chung sei kim

Verified customer

Submitted Jun 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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