Anti-Clostridium botulinum A Toxoid antibody [B29M] (ab37344)

Overview

  • Product nameAnti-Clostridium botulinum A Toxoid antibody [B29M]
    See all Clostridium botulinum A Toxoid primary antibodies
  • Description
    Mouse monoclonal [B29M] to Clostridium botulinum A Toxoid
  • SpecificityClostridium botulinum Type A Neurotoxin Heavy Chain.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Clostridium botulinum. Not yet tested in other specie.
  • Immunogen

    Synthetic peptide:

    DRVYINVVVKNKEYRLATN

    , corresponding to amino acids 1177-1195 of Clostridium botulinum A Toxoid

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesab37344 was purified from ascites by euglobulin precipitation.
  • ClonalityMonoclonal
  • Clone numberB29M
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 149 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceClostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
    • Alternative names
      • Atx antibody
      • BotA antibody
      • BOTOX antibody
      • Botulinum neurotoxin type A antibody
      • Botulism A toxoid antibody
      see all

    References for Anti-Clostridium botulinum A Toxoid antibody [B29M] (ab37344)

    This product has been referenced in:
    • Tok JB & Fischer NO Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin. Chem Commun (Camb) :1883-5 (2008). Read more (PubMed: 18401506) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I have been in contact with the originator of this antibody, and this is the response I received: Most antibody manufacturers use an extinction coefficient of 1.35-1.4 for IgG as well as IgM. There was some theory years ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"