Anti-Clostridium botulinum A Toxoid antibody [KBA211] (ab20592)

Overview

  • Product nameAnti-Clostridium botulinum A Toxoid antibody [KBA211]
    See all Clostridium botulinum A Toxoid primary antibodies
  • Description
    Mouse monoclonal [KBA211] to Clostridium botulinum A Toxoid
  • SpecificityThere is no cross-reactivity with B and E toxoids in ELISA.
  • Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with Clostridium botulinum. Not yet tested in other species.
  • Immunogen

    Full length protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberKBA211
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceClostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
    • Alternative names
      • Atx antibody
      • BotA antibody
      • BOTOX antibody
      • Botulinum neurotoxin type A antibody
      • Botulism A toxoid antibody
      see all

    References for Anti-Clostridium botulinum A Toxoid antibody [KBA211] (ab20592)

    ab20592 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    The preparation may not be the same for all type A toxin antibodies because the antibodies may be from different sources. If you provide me with a list of the catalogue numbers of the ones you are interested, I can l...

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    Thank you for contacting Abcam.


    Below please find the protocol used with these monoclonal antibodies:


    1. Primary antibodies are coated on plate at concentration 5 ug/ml (in PBS, pH 7.4) overnight at + 4 °C
    2. Washin...

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    Thank you for your enquiry. Thank you for explaining your approach. Your approach is certainly very interesting for the detection of BTA; and direct or sandwich ELISA would certainly be the assay that I would employ to determine the immmune response...

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    Thank you for your enquiry. To clarify can you tell me whether you are interested in quantifying the response to the BT-A injections that you intend to perform using sandwich ELISA. Would this be to detect an early IgM response or the subsequent Ig...

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    Thank you very much for your enquiry. I'm very sorry for the delay in responding to you. Thank you for your patience. There is no problems in ordering 15- 20 units at a time for ab2029, ab20592, ab22037, ab22041, as these are quite small amounts...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"