Anti-Clostridium botulinum A Toxoid antibody [KBA468] (HRP) (ab22507)

Overview

  • Product name
    Anti-Clostridium botulinum A Toxoid antibody [KBA468] (HRP)
    See all Clostridium botulinum A Toxoid primary antibodies
  • Description
    Mouse monoclonal [KBA468] to Clostridium botulinum A Toxoid (HRP)
  • Conjugation
    HRP
  • Specificity
    ab22507 recognises Clostridium botulinum A Toxoid in ELISA but does not cross-react with B and E toxoids. Reactivity: 100% A Toxoid 1.0% B Toxoid 1.2% E Toxoid
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Formaldehyde inactivated full-length C. botulinum A toxoid protein. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with Clostridium botulinum A Toxoid

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, 10mg/ml BSA. pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    KBA468
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
    • Alternative names
      • Atx antibody
      • BotA antibody
      • BOTOX antibody
      • Botulinum neurotoxin type A antibody
      • Botulism A toxoid antibody
      see all

    References

    ab22507 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We have used the pair ab22507 - ab20592 successfully in sandwich ELISA using the below protocol:
    1.Coat ab20592 on the plate at concentration of 5 ug/ml (in PBS, pH 7.4) overnight at + 4C
    2. Wash three times using 200 ul of PBST per well, 1 m...

    Read More

    Thank you for your reply.

    The preparation may not be the same for all type A toxin antibodies because the antibodies may be from different sources. If you provide me with a list of the catalogue numbers of the ones you are interested, I can l...

    Read More

    Thank you for contacting Abcam.


    Below please find the protocol used with these monoclonal antibodies:


    1. Primary antibodies are coated on plate at concentration 5 ug/ml (in PBS, pH 7.4) overnight at + 4 °C
    2. Washin...

    Read More

    Thank you for contacting us.

    We do have an ab22507Anti-Clostridium botulinum A Toxoid antibody which is indeed conjugated to HRP (http://www.abcam.com/Clostridium-botulinum-A-Toxoid-antibody-KBA468-HRP-ab22507.html). I believe that the mis...

    Read More

    Thank you for contacting us. I can confirm that the product ab22507 is actually an unconjugated antibody. This antibody has mistakenly published as HRP conjugated product. I apologize for this error which will be corrected soon. You can purchase th...

    Read More

    Thank you for contacting us. I can confirm that I have logged your inquiry in our system and now waiting a reply for lab. I will get back to you. Many thanks for having patience!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up