Anti-Clostridium botulinum E Toxoid antibody [209F2] (ab40787)

Overview

  • Product name
    Anti-Clostridium botulinum E Toxoid antibody [209F2]
  • Description
    Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
  • Specificity
    BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
  • Tested applications
    Suitable for: ICC/IF, Inhibition Assaymore details
  • Immunogen

    Synthetic peptide analogue of the amino terminal region of BoTox-E.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Sterile filtered culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    209F2
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Inhibition Assay
  • Application notes
    IF: 1/50.
    This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.

    Inhib: Use at an assay dependent dilution.
    This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
    • Cellular localization
      Secreted
    • Alternative names
      • BoNT/E antibody
      • Bontoxilysin e antibody
      • Botulinum neurotoxin type e non toxic component antibody
      • Botulinum neurotoxin type e precursor antibody
      • Botulism E toxoid antibody
      • Ent120 antibody
      see all

    References

    This product has been referenced in:
    • Shu Y  et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). Read more (PubMed: 18171919) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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