Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)

Overview

  • Product name
    Anti-Clostridium botulinum Toxin B antibody [GR-3G7]
  • Description
    Mouse monoclonal [GR-3G7] to Clostridium botulinum Toxin B
  • Specificity
    This antibody reacts with Clostridium botulinum Toxin B.
  • Tested applications
    Suitable for: Flow Cyt, Competitive ELISAmore details
  • Species reactivity
    Reacts with Clostridium botulinum.
  • Immunogen

    Genetic immunisation with cDNA encoding Clostridium botulinum Toxin B.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    GR-3G7
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Competitive ELISA 1/200 - 1/400.

Target

  • Relevance
    Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the 76 Gln | Phe 77 bond of synaptobrevin 2.
  • Cellular localization
    Secreted
  • Alternative names
    • BoNT/B antibody
    • Bontoxilysin B antibody
    • BontoxilysinB antibody
    • bot B antibody
    • botB antibody
    • Botulinum neurotoxin type B antibody
    see all

Anti-Clostridium botulinum Toxin B antibody [GR-3G7] images

  • BOSC23 cells were transiently trans-fected with an expression vector encoding either Clostridium botulinum Toxin B (red curve) or an irrelevant protein (control transfectant). Binding of ab59715 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Clostridium botulinum Toxin B transfected cells.

References for Anti-Clostridium botulinum Toxin B antibody [GR-3G7] (ab59715)

ab59715 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question and for your patience while I have been in touch with the lab about ab59715. This antibody was raised against the light chain and has only been tested for reactivity with the light chain, so it most...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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