The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
1/2000 - 1/5000.
Clostridium difficile Cell Surface Protein contains a CW_binding_2 domain (Putative cell wall binding repeat 2) and a MurNAc-LAA domain: N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 18.104.22.168). It is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino. Cell Surface Protein is highly similar to CD1035.
Cell surface protein (N acetylmuramoyl L alanine amidase) antibody