Anti-Clostridium difficile Toxin A antibody [PCG4] (ab19953)

Overview

  • Product name
    Anti-Clostridium difficile Toxin A antibody [PCG4]
    See all Clostridium difficile Toxin A primary antibodies
  • Description
    Mouse monoclonal [PCG4] to Clostridium difficile Toxin A
  • Specificity
    C. difficile Toxin A. Does not cross react with C. difficile Toxin B
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Clostridium difficile
  • Immunogen

    Full length protein (C. difficile).

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    >98% pure (SDS-PAGE). Protein G chromatography.
  • Clonality
    Monoclonal
  • Clone number
    PCG4
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 308 kDa. PubMed: 24244530
ICC/IF Use at an assay dependent concentration. PubMed: 21266163
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • tcdA antibody
      • toxA antibody

    References

    This product has been referenced in:
    • Darkoh C  et al. Accessory Gene Regulator-1 Locus Is Essential for Virulence and Pathogenesis of Clostridium difficile. MBio 7:N/A (2016). WB . Read more (PubMed: 27531912) »
    • Dhillon HS  et al. Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B. Biomol Detect Quantif 10:2-8 (2016). Read more (PubMed: 27990343) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    I can confirm that the starting material of this product was ascites. Each lot of ascites is tested against C. difficile Toxin A (purified from bacterial broth growth) coated on the microtiter plate. Ascites was tested as a dilution series followed by ...

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    Thank you for your enquiry and your interest in our products.
    I can confirm that this antibody (ab19953:Anti-Clostridium difficile Toxin A antibody [PCG4]) was launched on June 2006.
    I hope this helps and if I can assist further, please do no...

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    Thank you for letting me know this. I have now updated the species list.

    If you would like to submit similar reviews for the other antibodies you have used please do. Each new review will earn you more Abpoints which can be used to claim mone...

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    I have now returned from the long weekend, I hope the delay in my reply has not affected your work.

    Thank you for providing me with this feedback of how you found the anti-C. difficile toxin antibodies to work. In regards to the anti-Clostrid...

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    Vielen Dank für Ihren Anruf.

    Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

    Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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