Anti-Clostridium difficile Toxin A antibody [PCG4] (ab19953)

Overview

  • Product nameAnti-Clostridium difficile Toxin A antibody [PCG4]
    See all Clostridium difficile Toxin A primary antibodies
  • Description
    Mouse monoclonal [PCG4] to Clostridium difficile Toxin A
  • SpecificityC. difficile Toxin A. Does not cross react with C. difficile Toxin B
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Clostridium difficile
  • Immunogen

    Full length protein (C. difficile).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notes>98% pure (SDS-PAGE). Protein G chromatography.
  • ClonalityMonoclonal
  • Clone numberPCG4
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 308 kDa. PubMed: 24244530
ICC/IF Use at an assay dependent concentration. PubMed: 21266163
ELISA Use at an assay dependent concentration.

Target

  • RelevanceClostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
  • Cellular localizationSecreted
  • Database links
    • Alternative names
      • tcdA antibody
      • toxA antibody

    References for Anti-Clostridium difficile Toxin A antibody [PCG4] (ab19953)

    This product has been referenced in:
    • Ransom EM  et al. Identification and Characterization of a Gene Cluster Required for Proper Rod Shape, Cell Division, and Pathogenesis in Clostridium difficile. J Bacteriol 196:2290-300 (2014). WB . Read more (PubMed: 24727226) »
    • Darkoh C  et al. Bile salt inhibition of host cell damage by clostridium difficile toxins. PLoS One 8:e79631 (2013). WB . Read more (PubMed: 24244530) »

    See all 3 Publications for this product

    Product Wall

    I can confirm that the starting material of this product was ascites. Each lot of ascites is tested against C. difficile Toxin A (purified from bacterial broth growth) coated on the microtiter plate. Ascites was tested as a dilution series followed by ...

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    Thank you for your enquiry and your interest in our products.
    I can confirm that this antibody (ab19953:Anti-Clostridium difficile Toxin A antibody [PCG4]) was launched on June 2006.
    I hope this helps and if I can assist further, please do no...

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    Thank you for letting me know this. I have now updated the species list.

    If you would like to submit similar reviews for the other antibodies you have used please do. Each new review will earn you more Abpoints which can be used to claim mone...

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    I have now returned from the long weekend, I hope the delay in my reply has not affected your work.

    Thank you for providing me with this feedback of how you found the anti-C. difficile toxin antibodies to work. In regards to the anti-Clostrid...

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    Vielen Dank für Ihren Anruf.

    Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

    Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"