Human Anti-Clostridium tetani toxin IgG ELISA Kit (ab108771)
Key features and details
- Sensitivity: 0.05 U/ml
- Range: 0.05 U/ml - 1 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Clostridium tetani toxin IgG ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Weak + Serum 24 0.56U/ml 7.1% Pos. Serum 24 1.24U/ml 4.9% Inter-assay Sample n Mean SD CV% Weak + Serum 10 0.22U/ml 11.3% Pos. Serum 10 0.54U/ml 7.7% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
< 0.05 U/ml -
Range
0.05 U/ml - 1 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.
A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Clostridium tetani toxin (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Clostridium tetani toxin anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Clostridium tetani toxin IgG Standard A – 0.0 U/mL Blue cap 1 x 2ml Clostridium tetani toxin IgG Standard B – 0.1 U/mL Green Cap 1 x 2ml Clostridium tetani toxin IgG Standard C – 0.5 U/mL Yellow cap 1 x 2ml Clostridium tetani toxin IgG Standard D – 1.0 U/mL Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108771 has not yet been referenced specifically in any publications.