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Rabbit polyclonal to CLPP
Recombinant fragment, corresponding to a region within amino acids 48-232 of Human CLPP (Q16740).
Human colon carcinoma tissue; Jurkat whole cell lysate.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney.
Belongs to the peptidase S14 family.
Information by UniProt
ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
ATP dependent protease ClpAP proteolytic subunit antibody
ATP dependent protease ClpAP proteolytic subunit human antibody
Western blot - Anti-CLPP antibody (ab126102)
Anti-CLPP antibody (ab126102) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 30 kDa 12% SDS Page
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody (ab126102)
ab126102, at 1/250 dilution, staining CLPP in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"