Recombinant Anti-CLPP antibody [EPR7133] (ab124822)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7133] to CLPP
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CLPP antibody [EPR7133]
See all CLPP primary antibodies -
Description
Rabbit monoclonal [EPR7133] to CLPP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CLPP.
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Positive control
- WB: Recombinant Human CLPP protein (ab124872), Fetal muscle, Saos-2, A431, 293T, K562, A549, and Mouse brain lysates; IHC-P: Human liver, Mouse kidney, and Rat stomach tissues IP: K562
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7133 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
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IP |
1/10 - 1/100.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa). |
IP
1/10 - 1/100. |
Target
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Function
Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. -
Tissue specificity
Predominantly expressed in skeletal muscle. Intermediate levels in heart, liver and pancreas. Low in brain, placenta, lung and kidney. -
Sequence similarities
Belongs to the peptidase S14 family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 8192 Human
- Entrez Gene: 53895 Mouse
- Entrez Gene: 301117 Rat
- Omim: 601119 Human
- SwissProt: Q16740 Human
- SwissProt: O88696 Mouse
- Unigene: 515092 Human
- Unigene: 287892 Mouse
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Alternative names
- ATP dependent protease ClpAP (E coli) proteolytic subunit antibody
- ATP dependent protease ClpAP proteolytic subunit antibody
- ATP dependent protease ClpAP proteolytic subunit human antibody
see all
Images
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All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 0.05 µg/ml (purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 30 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
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ab124822 (purified) at 1:20 dilution (2µg) immunoprecipitating CLPP in K562 whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab124822 & K562 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution (unpurified)
Lane 1 : Fetal muscle lysate
Lane 2 : Saos-2 (Human osteosarcoma cell line) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 5 : K562 cell lysate
Lane 6 : A549 (Human lung carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab124822 has been referenced in 36 publications.
- Chattopadhyay M et al. The portrait of liver cancer is shaped by mitochondrial genetics. Cell Rep 38:110254 (2022). PubMed: 35045282
- Zhao Y et al. ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. Nat Commun 13:1121 (2022). PubMed: 35236834
- Meseguer S & Rubio MP mt tRFs, New Players in MELAS Disease. Front Physiol 13:800171 (2022). PubMed: 35273517
- Salazar-Petres E et al. Placental structure, function, and mitochondrial phenotype relate to fetal size in each fetal sex in mice†. Biol Reprod 106:1292-1311 (2022). PubMed: 35293971
- Wei B et al. Anti-infective therapy using species-specific activators of Staphylococcus aureus ClpP. Nat Commun 13:6909 (2022). PubMed: 36376309