Overview

  • Product nameAnti-CLPX antibody
    See all CLPX primary antibodies
  • Description
    Rabbit polyclonal to CLPX
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GRLGTFETQILQRAPLRSFTETPAYFASKDGISKDGSGDGNKKSASEGSS KKSGSGNSGK GGNQLRCPKCGDLCTHVETFVSSTRFVKCEKCHH, corresponding to amino acids 39-132 of Human CLPX. (O76031).

  • Positive control
    • Human cell line U 2 OS. Human plasma. Human testis.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • FunctionATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
  • Tissue specificityHigher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
  • Sequence similaritiesBelongs to the ClpX chaperone family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
    • ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
    • AU014732 antibody
    • Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody
    • Caseinolytic peptidase X (E.coli) antibody
    • Caseinolytic protease X antibody
    • clpX antibody
    • ClpX caseinolytic peptidase X homolog antibody
    • ClpX caseinolytic protease X homolog antibody
    • CLPX_HUMAN antibody
    • E330029I21 antibody
    • Energy dependent regulator of proteolysis antibody
    see all

Anti-CLPX antibody images

  • ab122644, at 1/50, staining CLPX in paraffin embedded Human testis tissue by Immunohistochemistry.
  • ab122644, at 4 µg/ml, staining Human cell line U 2 OS cells by Immunofluorescence.

References for Anti-CLPX antibody (ab122644)

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - other (HeLa)
Specification HeLa
Total protein in input 500 µg
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Submitted Jun 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"