Overview

  • Product name
  • Description
    Rabbit polyclonal to CLSTN1
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CLSTN1 (N terminal). The immunogen is located between aa 100 and 200 of the human protein.
    Database link: O94985

Properties

Applications

Our Abpromise guarantee covers the use of ab73141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 110 kDa.

Target

  • Function
    Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals.
  • Tissue specificity
    Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level).
  • Sequence similarities
    Contains 2 cadherin domains.
  • Domain
    The cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca(2+).
  • Post-translational
    modifications
    Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection. Cell junction > synapse > postsynaptic cell membrane. Nucleus. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alc alpha antibody
    • Alc-alpha antibody
    • Alcadein alpha 1 antibody
    • Alcadein alpha antibody
    • Alcadein-alpha antibody
    • alcalpha1 antibody
    • alcalpha2 antibody
    • Alzheimer related cadherin like protein antibody
    • Alzheimer-related cadherin-like protein antibody
    • C-terminal fragment 1-alpha antibody
    • Cadherin related family member 12 antibody
    • Calsyntenin 1 antibody
    • Calsyntenin1 antibody
    • CDHR12 antibody
    • CLSTN 1 antibody
    • Clstn1 antibody
    • CS1 antibody
    • CSTN1 antibody
    • CSTN1_HUMAN antibody
    • CTF1-alpha antibody
    • FLJ32258 antibody
    • KIAA0911 antibody
    • Non classical cadherin XB31alpha antibody
    • Non classical cadherin XB31alpha1 antibody
    • Non-classical cadherin XB31alpha antibody
    • PIK3CD antibody
    • SAlc-alpha antibody
    • XB31alpha antibody
    see all

References

ab73141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NEURONAL)
Specification
NEURONAL
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (neuronal)
Loading amount
25 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NEURONAL)
Specification
NEURONAL
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
25 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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