The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceCLSTN2 (Alcadein gamma/Calsyntenin 2) is a member of the novel calsyntenin family of proteins that have been found to be localized in the postsynaptic membrane of excitatory central nervous system (CNS) synapses. It may play a role in postsynaptic Ca2+ signaling. Calsyntenin 2 structure is very similar to calsyntenin 1 in it's extracellular parts, but varies considerably in it's cytoplasmic segment.
Cellular localizationMembrane; single-pass type I membrane protein