The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceCLSTN3 may modulate calcium-mediated postsynaptic signals. It forms a complex with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (by similarity).
Cellular localizationCell Membrane (Single-pass type I membrane protein), Endoplasmic reticulum and Golgi Apparatus.