Overview

  • Product nameAnti-Clusterin antibody
    See all Clusterin primary antibodies
  • Description
    Goat polyclonal to Clusterin
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    EKALQEYRKKHREE

    with a Cysteine residue linker, corresponding to C terminal amino acids 436-449 of Human Apolipoprotein J (NP_001822.2).

  • Positive control
    • Human Testis tissue; Human colon lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 52 kDa.
ELISA 1/1 - 1/32000.

Target

  • FunctionIsoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • Tissue specificityDetected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • Sequence similaritiesBelongs to the clusterin family.
  • Post-translational
    modifications
    Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • Cellular localizationSecreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 antibody
    • AAG 4 antibody
    • AAG4 antibody
    • Aging associated protein 4 antibody
    • Aging-associated gene 4 protein antibody
    • AI893575 antibody
    • APO J antibody
    • Apo-J antibody
    • APOJ antibody
    • ApoJalpha antibody
    • ApoJbeta antibody
    • Apolipoprotein J antibody
    • ApolipoproteinJ antibody
    • CLI antibody
    • CLU antibody
    • CLU1 antibody
    • CLU2 antibody
    • CLUS_HUMAN antibody
    • Clusterin alpha chain antibody
    • Clusterin antibody
    • Clusterin beta chain antibody
    • Complement associated protein SP 40 40 antibody
    • Complement associated protein SP 40 antibody
    • Complement associated protein SP40 antibody
    • Complement cytolysis inhibitor a chain antibody
    • Complement cytolysis inhibitor antibody
    • Complement cytolysis inhibitor b chain antibody
    • Complement lysis inhibitor antibody
    • Complement-associated protein SP-40 antibody
    • D14Ucla3 antibody
    • Dimeric acid glycoprotein antibody
    • Glycoprotein 80 antibody
    • Glycoprotein III antibody
    • GP80 antibody
    • Ku70-binding protein 1 antibody
    • KUB 1 antibody
    • KUB1 antibody
    • MGC24903 antibody
    • NA1/NA2 antibody
    • RATTRPM2B antibody
    • SGP 2 antibody
    • SGP2 antibody
    • SP 40 antibody
    • SP40 antibody
    • Sugp-2 antibody
    • Sulfated glycoprotein 2 antibody
    • Testosterone repressed prostate message 2 antibody
    • Testosterone-repressed prostate message 2 antibody
    • TRPM 2 antibody
    • TRPM-2 antibody
    • TRPM2 antibody
    • TRPM2B antibody
    • Trpmb antibody
    see all

Anti-Clusterin antibody images

  • ab104652, at 2.5 µg/ml, staining Apolipoprotein J in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Clusterin antibody (ab104652) at 0.3 µg/ml + Human colon lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa

References for Anti-Clusterin antibody (ab104652)

ab104652 has not yet been referenced specifically in any publications.

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