Anti-Clusterin antibody (ab39991)
Key features and details
- Goat polyclonal to Clusterin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Clusterin antibody
See all Clusterin primary antibodies -
Description
Goat polyclonal to Clusterin -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Clusterin (C terminal).
Sequence:(C)EKALQEYRKKHREE
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Positive control
- WB: Human Colon lysate; ICC: HeLa cells; IHC-P: Human tonsil tissue section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 60 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
Tissue specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
Sequence similarities
Belongs to the clusterin family. -
Post-translational
modificationsIsoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
Cellular localization
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 1191 Human
- Omim: 185430 Human
- SwissProt: P10909 Human
- Unigene: 436657 Human
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Alternative names
- 40 antibody
- AAG 4 antibody
- AAG4 antibody
see all
Images
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Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, staining Clusterin with ab39991 at 10µg/ml. Cells were permeablized with 0.15% Triton. DAPI was used as a nuclear counterstain.
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Anti-Clusterin antibody (ab39991) at 0.3 µg/ml + Human Colon lysate, 35 µg protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 60 kDa
Datasheets and documents
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Datasheet download
References (4)
ab39991 has been referenced in 4 publications.
- Islam K et al. Development of a Label-Free Immunosensor for Clusterin Detection as an Alzheimer's Biomarker. Sensors (Basel) 18:N/A (2018). PubMed: 29361679
- Shafie IN et al. The chaperone protein clusterin may serve as a cerebrospinal fluid biomarker for chronic spinal cord disorders in the dog. Cell Stress Chaperones 19:311-20 (2014). PubMed: 23990410
- Chenau J et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960
- Choe L et al. 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment for Alzheimer's disease. Proteomics 7:3651-60 (2007). PubMed: 17880003