Anti-Clusterin antibody (ab69644)

Overview

  • Product nameAnti-Clusterin antibody
    See all Clusterin primary antibodies
  • Description
    Rabbit polyclonal to Clusterin
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Clusterin (C terminal).
    (Peptide available as ab139424)

  • Positive control
    • HeLa and A549 cell extracts, HeLa cells and human brain tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Clusterin peptide (ab139424).
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionIsoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
  • Tissue specificityDetected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
  • Sequence similaritiesBelongs to the clusterin family.
  • Post-translational
    modifications
    Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
    Polyubiquitinated, leading to proteasomal degradation.
    Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
  • Cellular localizationSecreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40 antibody
    • AAG 4 antibody
    • AAG4 antibody
    • Aging associated protein 4 antibody
    • Aging-associated gene 4 protein antibody
    • AI893575 antibody
    • APO J antibody
    • Apo-J antibody
    • APOJ antibody
    • ApoJalpha antibody
    • ApoJbeta antibody
    • Apolipoprotein J antibody
    • ApolipoproteinJ antibody
    • CLI antibody
    • CLU antibody
    • CLU1 antibody
    • CLU2 antibody
    • CLUS_HUMAN antibody
    • Clusterin alpha chain antibody
    • Clusterin antibody
    • Clusterin beta chain antibody
    • Complement associated protein SP 40 40 antibody
    • Complement associated protein SP 40 antibody
    • Complement associated protein SP40 antibody
    • Complement cytolysis inhibitor a chain antibody
    • Complement cytolysis inhibitor antibody
    • Complement cytolysis inhibitor b chain antibody
    • Complement lysis inhibitor antibody
    • Complement-associated protein SP-40 antibody
    • D14Ucla3 antibody
    • Dimeric acid glycoprotein antibody
    • Glycoprotein 80 antibody
    • Glycoprotein III antibody
    • GP80 antibody
    • Ku70-binding protein 1 antibody
    • KUB 1 antibody
    • KUB1 antibody
    • MGC24903 antibody
    • NA1/NA2 antibody
    • RATTRPM2B antibody
    • SGP 2 antibody
    • SGP2 antibody
    • SP 40 antibody
    • SP40 antibody
    • Sugp-2 antibody
    • Sulfated glycoprotein 2 antibody
    • Testosterone repressed prostate message 2 antibody
    • Testosterone-repressed prostate message 2 antibody
    • TRPM 2 antibody
    • TRPM-2 antibody
    • TRPM2 antibody
    • TRPM2B antibody
    • Trpmb antibody
    see all

Anti-Clusterin antibody images

  • ab69644 at 1/500 dilution staining Apolipoprotein J in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab69644 at 1/50 dilution staining Apolipoprotein J in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Clusterin antibody (ab69644) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : A549 extracts
    Lane 3 : A549 extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Clusterin antibody (ab69644)

ab69644 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The blocking peptide for ab69644 is now available as ab139424. I hope this helps, please let me know if you need any additional information or assistance.

That is super news. I am really pleased the alternative product worked well. As you have the images available I wonder whether you would like to submit an Abreview for ab69644? We currently have no Abreviews so will be eligible for extra points. Ab...

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