Overview

  • Product nameAnti-Clusterin antibody
    See all Clusterin primary antibodies
  • Description
    Goat polyclonal to Clusterin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Clusterin aa 435-448 (C terminal).
    Sequence:

    C-EKALQEYRRKSRAE

  • Positive control
    • Mouse Brain and Testis lysates; Rat Brain and Ovary lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab79280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIsoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
    • Tissue specificityDetected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
    • Sequence similaritiesBelongs to the clusterin family.
    • Post-translational
      modifications
      Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
      Polyubiquitinated, leading to proteasomal degradation.
      Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
    • Cellular localizationSecreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40 antibody
      • AAG 4 antibody
      • AAG4 antibody
      • Aging associated protein 4 antibody
      • Aging-associated gene 4 protein antibody
      • AI893575 antibody
      • APO J antibody
      • Apo-J antibody
      • APOJ antibody
      • ApoJalpha antibody
      • ApoJbeta antibody
      • Apolipoprotein J antibody
      • ApolipoproteinJ antibody
      • CLI antibody
      • CLU antibody
      • CLU1 antibody
      • CLU2 antibody
      • CLUS_HUMAN antibody
      • Clusterin alpha chain antibody
      • Clusterin antibody
      • Clusterin beta chain antibody
      • Complement associated protein SP 40 40 antibody
      • Complement associated protein SP 40 antibody
      • Complement associated protein SP40 antibody
      • Complement cytolysis inhibitor a chain antibody
      • Complement cytolysis inhibitor antibody
      • Complement cytolysis inhibitor b chain antibody
      • Complement lysis inhibitor antibody
      • Complement-associated protein SP-40 antibody
      • D14Ucla3 antibody
      • Dimeric acid glycoprotein antibody
      • Glycoprotein 80 antibody
      • Glycoprotein III antibody
      • GP80 antibody
      • Ku70-binding protein 1 antibody
      • KUB 1 antibody
      • KUB1 antibody
      • MGC24903 antibody
      • NA1/NA2 antibody
      • RATTRPM2B antibody
      • SGP 2 antibody
      • SGP2 antibody
      • SP 40 antibody
      • SP40 antibody
      • Sugp-2 antibody
      • Sulfated glycoprotein 2 antibody
      • Testosterone repressed prostate message 2 antibody
      • Testosterone-repressed prostate message 2 antibody
      • TRPM 2 antibody
      • TRPM-2 antibody
      • TRPM2 antibody
      • TRPM2B antibody
      • Trpmb antibody
      see all

    Anti-Clusterin antibody images

    • Anti-Clusterin antibody (ab79280) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg

      Predicted band size : 52 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
      The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857).

    References for Anti-Clusterin antibody (ab79280)

    ab79280 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"