Overview

  • Product nameAnti-Clusterin antibody [EPR2911]
    See all Clusterin primary antibodies
  • Description
    Rabbit monoclonal [EPR2911] to Clusterin
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Clusterin aa 400-500.

  • Positive control
    • Human tonsil, Fetal brain & Human plasma lysates, Human testis tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionIsoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
    • Tissue specificityDetected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
    • Sequence similaritiesBelongs to the clusterin family.
    • Post-translational
      modifications
      Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
      Polyubiquitinated, leading to proteasomal degradation.
      Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
    • Cellular localizationSecreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40 antibody
      • AAG 4 antibody
      • AAG4 antibody
      • Aging associated protein 4 antibody
      • Aging-associated gene 4 protein antibody
      • AI893575 antibody
      • APO J antibody
      • Apo-J antibody
      • APOJ antibody
      • ApoJalpha antibody
      • ApoJbeta antibody
      • Apolipoprotein J antibody
      • ApolipoproteinJ antibody
      • CLI antibody
      • CLU antibody
      • CLU1 antibody
      • CLU2 antibody
      • CLUS_HUMAN antibody
      • Clusterin alpha chain antibody
      • Clusterin antibody
      • Clusterin beta chain antibody
      • Complement associated protein SP 40 40 antibody
      • Complement associated protein SP 40 antibody
      • Complement associated protein SP40 antibody
      • Complement cytolysis inhibitor a chain antibody
      • Complement cytolysis inhibitor antibody
      • Complement cytolysis inhibitor b chain antibody
      • Complement lysis inhibitor antibody
      • Complement-associated protein SP-40 antibody
      • D14Ucla3 antibody
      • Dimeric acid glycoprotein antibody
      • Glycoprotein 80 antibody
      • Glycoprotein III antibody
      • GP80 antibody
      • Ku70-binding protein 1 antibody
      • KUB 1 antibody
      • KUB1 antibody
      • MGC24903 antibody
      • NA1/NA2 antibody
      • RATTRPM2B antibody
      • SGP 2 antibody
      • SGP2 antibody
      • SP 40 antibody
      • SP40 antibody
      • Sugp-2 antibody
      • Sulfated glycoprotein 2 antibody
      • Testosterone repressed prostate message 2 antibody
      • Testosterone-repressed prostate message 2 antibody
      • TRPM 2 antibody
      • TRPM-2 antibody
      • TRPM2 antibody
      • TRPM2B antibody
      • Trpmb antibody
      see all

    Anti-Clusterin antibody [EPR2911] images

    • ab92548 staining Apolipoprotein J in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 1% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • ab92548 at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
    • All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

      Lane 1 : Human tonsil tissue lysate
      Lane 2 : Fetal brain tissue lysate
      Lane 3 : Human Plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 52 kDa

    References for Anti-Clusterin antibody [EPR2911] (ab92548)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary astrocyte culture)
    Permeabilization Yes - 0.1M PBS with 1% Triton X
    Specification Primary astrocyte culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Methanol
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 06 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain)
    Antigen retrieval step None
    Permeabilization Yes - 0.1 M PBS with 1% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 29 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 30 µg
    Specification Brain lysate
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"