Recombinant
RabMAb

Anti-CMAS antibody [EPR10485] (ab157189)

Overview

  • Product name
    Anti-CMAS antibody [EPR10485]
    See all CMAS primary antibodies
  • Description
    Rabbit monoclonal [EPR10485] to CMAS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human CMAS (UniProt: Q8NFW8).

  • Positive control
    • RAW264.7, Human fetal brain, HeLa and 293T lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 48 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).
    • Tissue specificity
      Ubiquitously expressed. Expressed in pancreas, kidney, liver, skeletal muscle, lung, placenta, brain, heart, colon, PBL, small intestine, ovary, testis, prostate, thymus and spleen.
    • Pathway
      Amino-sugar metabolism; N-acetylneuraminate metabolism.
    • Sequence similarities
      Belongs to the CMP-NeuNAc synthase family.
    • Domain
      The BC2 (basic cluster 2) motif is necessary and sufficient for the nuclear localization and contains the catalytic active site. The localization in the nucleus is however not required for the enzyme activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMAS antibody
      • CMP N acetylneuraminic acid synthase antibody
      • CMP Neu5Ac synthetase antibody
      • CMP NeuNAc synthetase antibody
      • CMP sialic acid synthetase antibody
      • CMP-N-acetylneuraminic acid synthase antibody
      • CMP-N-acetylneuraminic acid synthetase antibody
      • CMP-NeuNAc synthase antibody
      • CSS antibody
      • Cytidine 5' monophosphate N acetylneuraminic acid synthetase antibody
      • Cytidine 5-prime-monophosphate N-acetylneuraminic acid synthetase antibody
      • Cytidine monophosphate N acetylneuraminic acid synthetase antibody
      • N acylneuraminate cytidylyltransferase antibody
      • N-acylneuraminate cytidylyltransferase antibody
      • NEUA_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CMAS antibody [EPR10485] (ab157189) at 1/1000 dilution

      Lane 1 : RAW264.7 cell lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 48 kDa

    • Flow cytometric analysis of 293 cells labeling CMAS with ab157189 at 1/100 (red) or a rabbit IgG (negative) (green).

    References

    ab157189 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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