The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.3 µg/ml. Predicted molecular weight: 69 kDa.
Tissue specificityHighly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ab115540, at 5µg/ml, staining CMG1 in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-CMG1 antibody (ab115540)
has not yet been referenced specifically in any publications.
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