Overview

  • Product nameAnti-CMG1 antibody
    See all CMG1 primary antibodies
  • Description
    Goat polyclonal to CMG1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    KTIVDALHSTSGN

    , corresponding to C terminal amino acids 588-600 of Human CMG1

  • Positive control
    • Lysates of cell line HEK293. Human liver tissue. Rat cortical neurons.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276).
ICC/IF Use a concentration of 2.5 µg/ml.

Target

  • Tissue specificityHighly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasmic vesicle. Intracellular vesicular compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • intraflagellar transport 74, Chlamydomonas, homolog of antibody
    • Capillary morphogenesis gene 1 protein antibody
    • Capillary morphogenesis protein 1 antibody
    • Ccdc2 antibody
    • CMG 1 antibody
    • CMG-1 antibody
    • Coiled-coil domain containing 2 antibody
    • Coiled-coil domain-containing protein 2 antibody
    • IFT74 antibody
    • IFT74_HUMAN antibody
    • intraflagellar transport 74 homolog (Chlamydomonas) antibody
    • intraflagellar transport 74 homolog antibody
    • Intraflagellar transport protein 74 homolog antibody
    see all

Anti-CMG1 antibody images

  • Anti-CMG1 antibody (ab38789) at 0.3 µg/ml + 293 lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab38789 at 5ug/ml staining CMG1 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab38789 at 2.5mg/ml staining CMG1 in primary rat cortical neurons by Immunocytochemistry/ Immunofluorecence. The image shows localization of CMG1 in cell body vesicles, along the neuronal processes. Image B is the control.

References for Anti-CMG1 antibody (ab38789)

This product has been referenced in:
  • Boldt K  et al. Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. J Clin Invest 121:2169-80 (2011). Read more (PubMed: 21606596) »
  • Momeni P  et al. Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. BMC Neurol 6:44 (2006). Read more (PubMed: 17166276) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"