The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276).
Use a concentration of 2.5 µg/ml.
Tissue specificityHighly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ab38789 at 5ug/ml staining CMG1 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab38789 at 2.5mg/ml staining CMG1 in primary rat cortical neurons by Immunocytochemistry/ Immunofluorecence. The image shows localization of CMG1 in cell body vesicles, along the neuronal processes. Image B is the control.
References for Anti-CMG1 antibody (ab38789)
This product has been referenced in:
Boldt K et al. Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. J Clin Invest121:2169-80 (2011).
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