Overview

  • Product nameAnti-CMKLR1 antibody
    See all CMKLR1 primary antibodies
  • Description
    Rabbit polyclonal to CMKLR1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from an internal sequence of Human CMKLR1.

  • Positive control
    • HepG2, HeLa or 293 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionOrphan receptor. Could be a chemotactic peptide receptor. May have a function in bone metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
  • Tissue specificityProminently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Developmental stageExpressed during bone development.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHEMERINR antibody
    • Chemokine like receptor 1 antibody
    • Chemokine-like receptor 1 antibody
    • CHEMR23 antibody
    • CMKLR1 antibody
    • CML1_HUMAN antibody
    • DEZ antibody
    • G protein coupled receptor ChemR23 antibody
    • G protein coupled receptor DEZ antibody
    • G-protein coupled receptor ChemR23 antibody
    • G-protein coupled receptor DEZ antibody
    • Orphan G protein coupled receptor antibody
    • Orphan G protein coupled receptor Dez antibody
    • Resolvin E1 receptor antibody
    • RVER1 antibody
    see all

Anti-CMKLR1 antibody images

  • All lanes : Anti-CMKLR1 antibody (ab64881) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells (5-30µg total protein).
    Lane 2 : extracts from HeLa cells (5-30µg total protein).
    Lane 3 : extracts from 293 cells (5-30µg total protein).
    Lane 4 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 26 kDa,34 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CMKLR1 antibody (ab64881)

This product has been referenced in:
  • Bauer S  et al. Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes. Endocrinology 152:26-35 (2011). WB ; Mouse . Read more (PubMed: 21084441) »
  • Campbell EL  et al. Resolvin E1-induced intestinal alkaline phosphatase promotes resolution of inflammation through LPS detoxification. Proc Natl Acad Sci U S A 107:14298-303 (2010). Read more (PubMed: 20660763) »

See all 3 Publications for this product

Product Wall

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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I am sorry that my suggestions did not improve your results with this antibody. Can you please provide an order number? I would be happy to process your request for a refund.


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Thank you for your phone call regarding ab64881 in WB.


As we had discussed, I would recommend increasing the dilution to see if this helps with the background on your blot.


If this does not improve your results I am happ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Adipocytes)
Loading amount 5 µg
Specification Adipocytes
Gel Running Conditions Reduced Denaturing (10% gel, semi-dry)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"