Overview

  • Product name
  • Description
    Mouse polyclonal to CMT
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to C terminal amino acids 185-284 of Human CMT. Target sequence used to make antibody:FNHVVQNEKS DTHTLVTSGV YAWFRHPSYV GWFYWSIGTQ VMLCNPICGV SYALTVWRFF RDRTEEEEIS LIHFFGEEYL EYKKRVPTGL PFIKGVKVDL.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • Purity
    Whole antiserum
  • Primary antibody notes
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 32 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Function
    Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
  • Tissue specificity
    Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
  • Sequence similarities
    Belongs to the isoprenylcysteine O-methyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700008E11Rik antibody
    • C80758 antibody
    • fcmt antibody
    • Gm13095 antibody
    • HSTE14 antibody
    • Icmt antibody
    • ICMT_HUMAN antibody
    • Isoprenylcysteine carboxylmethyltransferase antibody
    • MGC118506 antibody
    • MGC39955 antibody
    • MGC95322 antibody
    • MST098 antibody
    • MSTP098 antibody
    • OTTMUSG00000010406 antibody
    • pcCMT antibody
    • PCMT antibody
    • PPMT antibody
    • Prenylated protein carboxyl methyltransferase antibody
    • Prenylcysteine carboxyl methyltransferase antibody
    • Protein-S-isoprenylcysteine O-methyltransferase antibody
    • RP23-421E12.4 antibody
    • STE14 antibody
    see all

Images

  • All lanes : Anti-CMT antibody (ab52738) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated, at 1/5000 dilution
    Lane 2 : Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution


    Predicted band size : 32 kDa

References

ab52738 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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