- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CNAP1 antibody
See all CNAP1 primary antibodies -
DescriptionRabbit polyclonal to CNAP1
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Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide derived from within residues 1350 to the C-terminus of Human CNAP1.
(Peptide available as ab34337.)
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Positive control
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab34338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa). |
Target
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RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
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Cellular localizationNuclear and cytoplasmic.
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Database links
- Entrez Gene: 9918 Human
- Entrez Gene: 68298 Mouse
- Omim: 615638 Human
- SwissProt: Q15021 Human
- SwissProt: Q8K2Z4 Mouse
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Alternative names
- 2810406C15Rik antibody
- 2810465G24Rik antibody
- CAP-D2 antibody
see all
Anti-CNAP1 antibody images
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Western blot - CNAP1 antibody (ab34338)Lane 1 : Marker
Lanes 2 - 4 : Anti-CNAP1 antibody (ab34338) at 1 µg/ml
Lane 1 : As above
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Lane 4 :A431 whole cell lysate (ab7909) at 20 µg
Secondary
Lanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 157 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CNAP1 antibody (ab34338)Image courtesy of Human Protein Atlas ab34338 staining CNAP1 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-CNAP1 antibody (ab34338)
ab34338 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"