- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CNAP1 antibody
See all CNAP1 primary antibodies -
DescriptionRabbit polyclonal to CNAP1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide derived from within residues 1350 to the C-terminus of Human CNAP1.
(Peptide available as ab34337.)
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Positive control
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab34338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa). |
Target
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RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
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Cellular localizationNuclear and cytoplasmic.
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Database links
- Entrez Gene: 9918 Human
- Entrez Gene: 68298 Mouse
- Omim: 615638 Human
- SwissProt: Q15021 Human
- SwissProt: Q8K2Z4 Mouse
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Alternative names
- 2810406C15Rik antibody
- 2810465G24Rik antibody
- CAP-D2 antibody
see all
Images
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_im1.jpg)
Western blot - CNAP1 antibody (ab34338)Lane 1 : Marker
Lanes 2-4 : Anti-CNAP1 antibody (ab34338) at 1 µg/ml
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Lane 4 :A431 whole cell lysate (ab7909) at 20 µg
Secondary
Lanes 2-4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 157 kDa
Observed band size: 150 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CNAP1 antibody (ab34338)Image courtesy of Human Protein Atlas ab34338 staining CNAP1 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
References
ab34338 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"