Anti-CNBP antibody (ab48027)

Overview

  • Product nameAnti-CNBP antibody
    See all CNBP primary antibodies
  • Description
    Goat polyclonal to CNBP
  • SpecificityNo crossreaction expected with CCHC domain containing 13.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus Monkey, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    GESGHLARECTIE

    , corresponding to C terminal amino acids 162-174 of Human CNBP.

  • Positive control
    • Human muscle lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notesELISA: 1/8000 (detection limit).
    WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSingle stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
    • Tissue specificityPresent in all tissues examined.
    • Involvement in diseaseDefects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
    • Sequence similaritiesContains 7 CCHC-type zinc fingers.
    • Cellular localizationCytoplasm. Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCHC type zinc finger nucleic acid binding protein antibody
      • Cellular nucleic acid binding protein antibody
      • Cellular nucleic acid-binding protein antibody
      • CNBP antibody
      • CNBP_HUMAN antibody
      • CNBP1 antibody
      • DM2 antibody
      • Erythroid differentiation related antibody
      • PROMM antibody
      • Proximal myotonic myopathy nucleic acid binding protein antibody
      • RNF163 antibody
      • Sterol regulatory element binding protein antibody
      • ZCCHC22 antibody
      • Zinc finger protein 273 antibody
      • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
      • Zinc finger protein 9 antibody
      • ZNF9 antibody
      see all

    Anti-CNBP antibody images

    • ICC/IF image of ab48027 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab48027 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer

      Secondary
      anti-goat-HRP at a 1/3000 dilution
      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 19 kDa
    • All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from 9L cells
      Lane 2 : Whole cell lysate prepared from PC12 cells
      Lane 3 : Whole cell lysate prepared from RIE-1 cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 6 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    • All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

      Lane 1 : Whole cell lysate prepared from MEF cells
      Lane 2 : Whole cell lysate prepared from F9 cells
      Lane 3 : Whole cell lysate prepared from GC-1 cells
      Lane 4 : Whole cell lysate prepared from NIH-3T3 cells

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-CNBP antibody (ab48027)

    ab48027 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse cell lines)
    Loading amount 20 µg
    Specification Mouse cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 23 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat cell lines)
    Loading amount 20 µg
    Specification Rat cell lines
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2012

    Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes f...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T HeLa U251 Jurkat U2OS)
    Loading amount 20 µg
    Specification 293T HeLa U251 Jurkat U2OS
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"